The one-step bonded, end-capped, amide-based polar-embedded stationary phase of Ascentis™ Express 2 μm RP-Amide provides, a stable, reversed phase packing with decreased hydrophobic character. Ascentis™ Express 2 μm RP-Amide can be used for basic, acidic, or neutral compounds with orthogonal selectivity from C18 or C8.